Antigen Affinity Purified Polyclonal Antibodies

Anti-Murine IL-7

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Product Details

Catalogue Number: 500-P57

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure recombinant Murine IL-7. Anti-Murine IL-7 specific antibody was purified by affinity chromatography employing immobilized Murine IL-7 matrix.

Immunogen: E.coli derived Recombinant Murine IL-7 (PeproTech catalog# 217-17)

Sandwich ELISA: To detect Murine IL-7 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Murine IL-7 (500-P57Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine IL-7.

Anti-Murine IL-7 Sandwich ELISA

Western Blot: To detect Murine IL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine IL-7 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Murine IL-7 Western Blot Reduced Anti-Murine IL-7 Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Gu, Y
Title
Forced co-expression of IL-21 and IL-7 in whole-cell cancer vaccines promotes antitumor immunity.
Citation
Scientific Reports; 6 pg32351
PubMed Id
First Author
Klooster, J P
Title
Enterocytes, fibroblasts and myeloid cells synergize in anti-bacterial and anti-viral pathways with IL22 as the central cytokine
Citation
Communications Biology; 4(1) pg631
PubMed Id