Antigen Affinity Purified Polyclonal Antibodies

Source: Polyclonal Goat

Preparation: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant Rat IL-1α. Anti-Rat IL-1α specific antibody was purified by affinity chromatography employing immobilized Rat IL-1α matrix.

Immunogen: E.coli derived Recombinant Rat IL-1α (PeproTech catalog# 400-01A)

Sandwich ELISA: To detect Rat IL-1α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Rat IL-1α (500-P180GBt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-1α.

Western Blot: To detect Rat IL-1α by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-1α is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.