Antigen Affinity Purified Polyclonal Antibodies

Anti-Rat MCP-1 (CCL2)

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Product Details

Catalogue Number: 500-P76

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat MCP-1(MCAF). Anti-Rat MCP-1(MCAF) specific antibody was purified by affinity chromatography employing immobilized Rat MCP-1(MCAF) matrix.

Immunogen: E.coli derived Recombinant Rat MCP-1 (CCL2) (PeproTech catalog# 400-12)

Sandwich ELISA: To detect Rat MCP-1(MCAF) by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Rat MCP-1(MCAF) (500-P76Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat MCP-1(MCAF).

Anti-Rat MCP-1 (CCL2) Sandwich ELISA

Western Blot: To detect Rat MCP-1(MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat MCP-1(MCAF) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Rat MCP-1 (CCL2) Western Blot Reduced Anti-Rat MCP-1 (CCL2) Western Blot Unreduced


Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Country Of Origin: USA

Not for human use.

Research Interest


First Author
Mao, L
Epothilone B impairs functional recovery after spinal cord injury by increasing secretion of macrophage colony-stimulating factor.
Cell death & disease; 8(11) pge3162
PubMed Id
First Author
Chen, X L
Angiotensin II induces monocyte chemoattractant protein-1 gene expression in rat vascular smooth muscle cells.
Circulation Research; 83(9) pg952-9
PubMed Id
First Author
Rius, C
Trans- but not cis-resveratrol impairs angiotensin-II-mediated vascular inflammation through inhibition of NF-κB activation and peroxisome proliferator-activated receptor-gamma upregulation.
Journal of immunology (Baltimore, Md. : 1950); 185(6) pg3718-27
PubMed Id