Biotinylated Polyclonal Antibodies

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hFGF-17. Anti-Human FGF-17 specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: E.coli derived Recombinant Human FGF-17 (PeproTech catalog# 100-27)

Direct ELISA: To detect Human FGF-17 by direct ELISA (using 100μl/well) a concentration of approximately 1.0μg/ml of this antibody is required. This biotinylated polyclonal antibody allows the detection of at least 2000-4000 pg/ml of Recombinant Human FGF-17.

Western Blot: To detect hFGF-17 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-17 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Note:

1mg will be provided as 2x500μg.