Biotinylated Anti-Human MCP-1 (CCL2) 0 ReviewsSubmit a Review Product Details Catalogue Number: 500-P34BT Description: Source: Polyclonal Rabbit Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMCP-1/MCAF. Anti-Human MCP-1/MCAF specific antibody was purified by affinity chromatography and then biotinylated. Immunogen: E.coli derived Recombinant Human MCP-1 (CCL2) (PeproTech catalog# 300-04) Sandwich ELISA: To detect hMCP-1/MCAF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human MCP-1/MCAF (500-P34) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hMCP-1/MCAF. Western Blot: To detect hMCP-1/MCAF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-1/MCAF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Note: 1mg will be provided as 2x500μg. crossreactivity: Human, Mouse References SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest COVID-19 Angiogenesis/Cardiovascular Cancer Chemotaxis Immune System Inflammation Neurobiology Wound Healing Allergy product.subtitle.recentcitations First Author Rius, C Title Trans- but not cis-resveratrol impairs angiotensin-II-mediated vascular inflammation through inhibition of NF-κB activation and peroxisome proliferator-activated receptor-gamma upregulation. Citation Journal of immunology (Baltimore, Md. : 1950); 185(6) pg3718-27 PubMed Id 20709957 First Author Lentsch, A B Title STAT4 and STAT6 regulate systemic inflammation and protect against lethal endotoxemia. Citation The Journal of Clinical Investigation; 108(10) pg1475-82 PubMed Id 11714739
