Biotinylated Polyclonal Antibodies

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMCP-4. Anti-Human MCP-4 specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: E.coli derived Recombinant Human MCP-4 (CCL13) (PeproTech catalog# 300-24)

Sandwich ELISA: To detect Human MCP-4 by sandwich ELISA (using 100μl/well) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human MCP-4 (CCL13) (500-P04) as a capture antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human MCP-4.

Western Blot: To detect Human MCP-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents the detection limit for Recombinant Human MCP-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Note:

1mg will be provided as 2x500μg.