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Biotinylated Polyclonal Antibodies

Biotinylated Anti-Human TNF-α (Polyclonal Goat)

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Detalles del Producto

Nùmero de Catalogo 500-P31AGBT
Descripcion
Biotinylated Anti-Human TNF-α (Polyclonal Goat)
 

Source: Polyclonal Goat

Preparation: Produced from sera of goats pre-immunized with highly pure recombinant Human TNF-α. Anti-Human TNF-α specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: E.coli-derived, 17.4 kDa Recombinant Human TNF-α (PeproTech catalog# 300-01A)

Sandwich ELISA: To detect Human TNF-α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human TNF-α (500-P31AG) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human TNF-α.

Western Blot: To detect Human TNF-α by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human TNF-α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Note:

1mg will be provided as 2x500μg.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

Primer autor
Bugueno, I M
Titulo
Porphyromonas gingivalis triggers the shedding of inflammatory endothelial microvesicles that act as autocrine effectors of endothelial dysfunction.
Citar
Scientific Reports; 10(1) pg1778
PudMed id