Biotinylated Anti-Murine Exodus-2 (CCL21) 0 ReviewsSubmit a Review Product Details Catalogue Number: 500-P114BT Description: Source: Polyclonal Rabbit Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mExodus-2. Anti-Murine Exodus-2 specific antibody was purified by affinity chromatography and then biotinylated. Immunogen: E.coli derived Recombinant Murine Exodus-2 (CCL21) (PeproTech catalog# 250-13) Sandwich ELISA: To detect mExodus-2 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Murine Exodus-2 (500-P114) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mExodus-2. Western Blot: To detect mExodus-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mExodus-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Note: 1mg will be provided as 2x500μg. crossreactivity: Mouse References SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest AIDS/HIV Chemotaxis Immune System Inflammation Wound Healing product.subtitle.recentcitations First Author Tsuboi, K Title Role of high endothelial venule-expressed heparan sulfate in chemokine presentation and lymphocyte homing. Citation Journal of immunology (Baltimore, Md. : 1950); 191(1) pg448-55 PubMed Id 23733868 First Author Yang, B Title Binding of lymphoid chemokines to collagen IV that accumulates in the basal lamina of high endothelial venules: its implications in lymphocyte trafficking. Citation Journal of immunology (Baltimore, Md. : 1950); 179(7) pg4376-82 PubMed Id 17878332