Biotinylated Anti-Murine GM-CSF 0 ReviewsSubmit a Review Product Details Catalogue Number: 500-P65BT Description: Source: Polyclonal Rabbit Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine GM-CSF. Anti-Murine GM-CSF-specific antibody was purified by affinity chromatography and then biotinylated. Immunogen: E.coli derived Recombinant Murine GM-CSF (PeproTech catalog# 315-03) Sandwich ELISA: To detect mGM-CSF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Murine GM-CSF (500-P65) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mGM-CSF. Western Blot: To detect mGM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mGM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Note: 1mg will be provided as 2x500μg. crossreactivity: Mouse References SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest COVID-19 Cancer Cell Culture Inflammation Proliferation Stem Cells & Differentiation Wound Healing product.subtitle.recentcitations First Author Lentsch, A B Title STAT4 and STAT6 regulate systemic inflammation and protect against lethal endotoxemia. Citation The Journal of Clinical Investigation; 108(10) pg1475-82 PubMed Id 11714739