Biotinylated Anti-Murine VEGF165 0 ReviewsSubmit a Review Product Details Catalogue Number: 500-P131BT Description: Source: Polyclonal Rabbit Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mVEGF. Anti-Murine VEGF specific antibody was purified by affinity chromatography and then biotinylated. Immunogen: E.coli-derived, 39.0 kDa Recombinant Murine VEGF165 (PeproTech catalog# 450-32) Sandwich ELISA: To detect mVEGF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Murine VEGF (500-P131) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mVEGF. Western Blot: To detect mVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mVEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Note: 1mg will be provided as 2x500μg. crossreactivity: Mouse References SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Angiogenesis/Cardiovascular Cancer Inflammation Proliferation product.subtitle.recentcitations First Author Almeida, S C Title Neoplastic transformation of T lymphocytes through transgenic expression of a virus host modification protein. Citation PLoS ONE; 7(4) pge34140 PubMed Id 22558084
