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Biotinylated Polyclonal Antibodies

Biotinylated Anti-Rat MCP-1 (CCL2)

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Detalles del Producto

Nùmero de Catalogo 500-P76BT
Descripcion
Biotinylated Anti-Rat MCP-1 (CCL2)
 

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat MCP-1(MCAF). Anti-Rat MCP-1(MCAF) specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: E.coli derived Recombinant Rat MCP-1 (CCL2) (PeproTech catalog# 400-12)

Sandwich ELISA: To detect Rat MCP-1(MCAF) by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Rat MCP-1(MCAF) (500-P76) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat MCP-1(MCAF).

Biotinylated Anti-Rat MCP-1 (CCL2) Sandwich ELISA

Western Blot: To detect Rat MCP-1(MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat MCP-1(MCAF) is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Note:

1mg will be provided as 2x500μg.

crossreactivity:
Rat
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

Primer autor
Rius, C
Titulo
Trans- but not cis-resveratrol impairs angiotensin-II-mediated vascular inflammation through inhibition of NF-κB activation and peroxisome proliferator-activated receptor-gamma upregulation.
Citar
Journal of immunology (Baltimore, Md. : 1950); 185(6) pg3718-27
PudMed id
Primer autor
Abu-Taha, M
Titulo
Menopause and ovariectomy cause a low grade of systemic inflammation that may be prevented by chronic treatment with low doses of estrogen or losartan.
Citar
Journal of immunology (Baltimore, Md. : 1950); 183(2) pg1393-402
PudMed id