Pre-Coated ELISA Kits

The three mammalian isoforms of TGF-β, TGF-β1, β2, and β3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility, as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes, including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes, which are stored at the cell surface and in the extracellular matrix. The release of the biologically active TGF-β isoform from a latent complex involves proteolytic processing of the complex and/or induction of conformational changes by proteins such as thrombospondin-1. The physiological role of TGF-β3 is still unknown, but its expression pattern suggests a role in the regulation of certain development processes. The BioGems Human TGF-β3 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure TGF-β3 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for TGF-β3. It contains sf21-expressed recombinant TGF-β3. The detection antibody is a biotinylated goat polyclonal antibody specific for TGF-β3. The kit is analytically validated with ready to use reagents.