Pre-Coated ELISA Kits

IFN-γ is an acid-labile interferon produced by CD4 and CD8 T lymphocytes, as well as activated NK cells. IFN-γ receptors are present in most immune cells, which respond to IFN-γ signaling by increasing the surface expression of class I MHC proteins. This promotes the presentation of antigen to T-helper (CD4+) cells. IFN-γ signaling in antigen-presenting, cells and antigen-recognizing B and T lymphocytes, regulates the antigen-specific phases of the immune response. Additionally, IFN-γ stimulates a number of lymphoid cell functions, including the anti-microbial and anti-tumor responses of macrophages, NK cells, and neutrophils. Human IFN-γ is species-specific and is biologically active only in human and primate cells. The BioGems Murine IFN-γ Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Murine IFN-γ with a 96-well strip plate that is pre-coated with rat monoclonal antibody specific for IFN-γ. It contains E.coli-expressed recombinant Murine IFN-γ. The detection antibody is a biotinylated goat polyclonal antibody specific for IFN-γ. The kit is analytically validated with ready to use reagents.