Media

PeproGrow™ hESC Embryonic Stem Cell Media

Detalles del Producto

Nùmero de Catalogo hESC
Descripcion

PeproGrow™ hESC is a serum- and phenol red-free medium of a complete, chemically-defined formulation designed for feeder-free maintenance and expansion of both human embryonic stem cells (hESCs) and human induced pluripotent stem cells (iPSCs) using BD Matrigel™ as a surface-coating matrix. This medium is intended for the culturing of hESCs and iPSCs in the undifferentiated, pluripotent state (SSEA4+/Oct4+), and demonstrates less than 15% spontaneous differentiation as indicated by flow cytometry. The proprietary formulation of the medium includes relevant growth factors, such as FGF2 (FGF-basic), but does not contain the insulin found in the majority of other hESC/iPSC media currently available on the market. PeproGrow™ hESC, which was designed and developed by PeproTech in collaboration with the Stem Cell Training Course at Rutgers University, is supplied as a 100mL, or 500mL, bottle of basal medium and a separate, lyophilized growth factor component.


Human Embryonic Stem Cell Basal Media and Growth Factor



PSC Immunophenotypes Are Maintained in PeproGrow hESC Medium


Human Embryonic Stem Cells (hESCs) and Induced Pluripotent Stem Cells (iPSCs) expanded in PeproGrow™ hESC Medium maintain an Oct4+/SSEA4+/Nanog+/Tra-1-60+/SSEA1- phenotype as shown by flow cytometry (A) and immunofluorescent microscopy (B). 


A.                                                                                                                                           B.

Flow Cytometry Human ESC Medium Immunofluorescent Microscopy hESC Medium




Pluripotent Stem Cells Grow and Proliferate Rapidly in PeproGrow™ hESC Medium

H1 Human ES Cells transduced with turboGFP-Neo driven by an Oct4 promoter and expanded on Matrigel proliferate rapidly when grown in PeproGrow™ hESC Medium.

hESC Rapid Proliferation

 

 

 

Note: The manual for this media can be found here

Cross Reactivity:
Human
Research Interest

Country Of Origin: USA

Not for human use.