Recombinant Human GDF-5 (BMP-14/CDMP-1) 0 ReviewsSubmit a Review Product Details Catalogue Number: 120-01 Description: GDF-5 is expressed in long bones during embryonic development and postnatally in articular cartilage. Mutations in the GDF-5 gene have been implicated in Hunter-Thompson type dwarfism and in Grebe Syndrome, which is characterized by short stature, extra digits, and short and deformed extremities. The mature and functional form of GDF-5 is a homodimer of two 120 amino-acid polypeptide chain (monomers) linked by a single disulfide bond. Each GDF-5 monomer is expressed as the C-terminal part of a precursor polypeptide, which also contains a 27 amino acid signal peptide and a 354 amino acid propeptide. This precursor undergoes intracellular dimerization, and upon secretion it is processed by a furin-type protease. Recombinant Human GDF-5 is a 27.0 kDa homodimeric disulfide-linked protein consisting of two 120 amino acid polypeptide chains. Source: E.coli Synonyms: Growth/Differentiation Factor-5, BMP-14, Cartilage-Derived Morphogenetic Protein-1 (CDMP-1) AA Sequence (monomer): APLATRQGKR PSKNLKARCS RKALHVNFKD MGWDDWIIAP LEYEAFHCEG LCEFPLRSHL EPTNHAVIQT LMNSMDPEST PPTCCVPTRL SPISILFIDS ANNVVYKQYE DMVVESCGCR Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: The ED50 was determined by its ability to induce alkaline phosphatase production by ATDC-5 chondrogenic cells is 1.0-2.0 µg/ml. Calculated Molecular Weight: 27 kDa Accession Number: P43026 Gene ID: 8200 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) crossreactivity: Human, Mouse, Sheep References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Angiogenesis/Cardiovascular Bone, Skeletal, Cartilage Cell Culture Immune System Stem Cells & Differentiation TGF-β Superfamily product.subtitle.recentcitations First Author Brown, W E Title Engineering large, anatomically shaped osteochondral constructs with robust interfacial shear properties Citation NPJ Regenerative Medicine; 6(1) pg42 PubMed Id 34362933 First Author Takei, Y Title Quality assessment tests for tumorigenicity of human iPS cell-derived cartilage. Citation Scientific Reports; 10(1) pg12794 PubMed Id 32732907 First Author Griffiths, R Title The Transcription Factor-microRNA Regulatory Network during hESC-chondrogenesis Citation Scientific Reports; 10(1) pg4744 PubMed Id 32179818
