Growth Factors & Cytokines

Recombinant Human IFN-γ

Product Details

Catalogue Number: 300-02
Description:
Recombinant Human IFN-γ

IFN-γ is an acid-labile interferon produced by CD4 and CD8 T lymphocytes as well as activated NK cells.  IFN-γ receptors are present in most immune cells, which respond to IFN-γ signaling by increasing the surface expression of class I MHC proteins.  This promotes the presentation of antigen to T-helper (CD4+) cells.  IFN-γ signaling in antigen-presenting cells, and antigen-recognizing B and T lymphocytes, regulates the antigen-specific phases of the immune response.  Additionally, IFN-γ stimulates a number of lymphoid cell functions, including the anti-microbial and anti-tumor responses of macrophages, NK cells, and neutrophils.  Human IFN-γ is species-specific and is biologically active only in human and primate cells.  Recombinant Human IFN-γ is a 16.8 kDa protein containing 144 amino acid residues.

Source: E.coli

Synonyms: Immune Interferon, type II interferon, T cell interferon, MAF

AA Sequence: MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Assay #1: Determined by its ability to induce STAT1/STAT2 activation in Human COLO 205 ISRE LUC reporter cells.

Assay #2: The ED50 determined by a cytotoxicity assay using HT-29 cells is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.

This product has been tested against the WHO standard. International Unit information can be found here.

Calculated Molecular Weight: 16.8 kDa

Accession Number: P01579.1

Gene ID: 3458

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
George, S
Title
Protocol to drive human monocyte-to-macrophage polarization in vitro using tumor conditioned media.
Citation
STAR Protocols; 3(4) pg101666
PubMed Id
First Author
Jiménez-Andrade, Y
Title
The Developmental Transcription Factor p63 Is Redeployed to Drive Allergic Skin Inflammation through Phosphorylation by p38α.
Citation
Journal of immunology (Baltimore, Md. : 1950); 208(12) pg2613-2621
PubMed Id
First Author
Fendereski, M
Title
Mouse Trophoblast Cells Can Provide IFN-Based Antiviral Protection to Embryonic Stem Cells via Paracrine Signaling.
Citation
Journal of immunology (Baltimore, Md. : 1950); 208(12) pg2761-2770
PubMed Id

product.subtitle.productreviews

Existing reviews
Confirmed dose response
Used in an experiment and saw a nice dose response effect
From:  Lucia | Date:  24/08/2021