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Growth Factors & Cytokines

Recombinant Human Noggin

Detalles del Producto

Nùmero de Catalogo 120-10C
Descripcion

Noggin belongs to a group of diffusible proteins that bind to ligands of the TGF-β family, and regulate their activity by inhibiting their access to signaling receptors. The interplay between TGF-β ligands and their natural antagonists has major biological significance during development processes, in which cellular response can vary considerably depending upon the local concentration of the signaling molecule. Noggin was originally identified as a BMP-4 antagonist whose action was critical for proper formation of the head and other dorsal structures.  Consequently, noggin has been shown to modulate the activities of other BMPs including BMP-2,-7,-13, and -14.  Targeted deletion of noggin in mice results in prenatal death, and a recessive phenotype displaying a severely malformed skeletal system. Conversely, transgenic mice over-expressing noggin in mature osteoblasts display impaired osteoblastic differentiation, reduced bone formation, and severe osteoporosis. Recombinant Human Noggin is a 46 kDa disulfide-linked homodimer consisting of two 205 amino acid polypeptide chains. Monomeric glycosylated noggin migrates at an apparent molecular weight of approximately 28.0-33.0 kDa by SDS PAGE analysis under reducing conditions.

Source: HEK293 cells

Synonyms: None

AA Sequence (monomer): QHYLHIRPAP SDNLPLVDLI EHPDPIFDPK EKDLNETLLR SLLGGHYDPG FMATSPPEDR PGGGGGAAGG AEDLAELDQL LRQRPSGAMP SEIKGLEFSE GLAQGKKQRL SKKLRRKLQM WLWSQTFCPV LYAWNDLGSR FWPRYVKVGS CFSKRSCSVP EGMVCKPSKS VHLTVLRWRC QRRGGQRCGW IPIQYPIISE CKCSC

Purity: ≥ 95% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by its ability to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The expected ED50 for this effect is 2.0-3.0 ng/ml of Noggin.

Calculated Molecular Weight: 46 kDa

Accession Number: Q13253

Gene ID: 9241

Note:

1mg will be provided as 2x500μg.

crossreactivity: Research Interest

Country Of Origin: USA

Not for human use.

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Comentarios existentes
It works well
It works well in organoid culture
De:  Eun Joo | Fecha:  08/07/2020
Necessary for organoid growth
Recombinant Human Noggin is one of the critical niche components for organoid growth media.
Delivered quickly.
De:  Kushtrim | Fecha:  09/07/2020
Human Noggin
Great product - works perfectly for organoid media. Shipped very quickly.
De:  Neel | Fecha:  21/07/2020
Excellent quality
We utilize H. Noggin for lung cancer organoid media and so far it has given us amazing results. The order was processed in a timely manner as well.
De:  Dora | Fecha:  24/07/2020
Good quality and good price
This product worked well for my project.  The price is reasonable and lower than most of vendors.
De:  Jin | Fecha:  26/08/2020
Recombinant Human Noggin for Organoid Media
Recombinant Human Noggin essential for organoid growth media. I used it for growing CRC organoids. Works very well and quick delivery. Recommend for any organoid media.
De:  Ilias | Fecha:  26/08/2020
good product
We routinely purchase Recombinant Human Noggin for our breast cancer organoid media. Peprotech has been easy to work with, quick to respond to questions, ship fast, and have had no issues with the product.
De:  Mark | Fecha:  26/08/2020
Rec Human Noggin
I have used this for the stem cell/organoid  culture for almost two years now. I am very happy with consistency of the product. In general, Peprotech has been a trust-able vendor for our lab needs.
De:  Arpit | Fecha:  26/08/2020
Perfect for organoids
We have purchased several products from peprotech to prepare our culture media for pancreatic organoids and the delivery was quick. This hNoggin works perfectly for us!
De:  Pilar | Fecha:  27/08/2020
Human Noggin for Lung Organoid Culture
Successfully cultured Lung Adenocarcinoma Organoid Culture. Mouse Noggin also works.
Very Quick Overnight Delivery.
Works great.
De:  Hardik | Fecha:  31/08/2020