Recombinant Human sFas Ligand 0 ComentariosSubmit a Review Detalles del Producto Nùmero de Catalogo 310-03H Descripcion Fas Ligand (FasL) is a member of the TNF superfamily that is expressed on the cell surface of activated T cells. Binding of FasL to Fas Receptor triggers apoptosis in Fas-bearing cells. FasL has the ability to kill T cells and activated B cells, which leads to down-regulation of the immune response. The mechanism of Fas-induced apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD, followed by processing of the pro-enzyme into active forms. These active caspases then cleave various cellular substrates, leading to the eventual cell death. Both human and murine sFasL are fully active on human and murine cells. Recombinant Human soluble Fas Ligand is a 17.9 kDa protein comprising the TNF-homologous region of FasL and contains an 8-residue N-terminal His-Tag. Source: CHO cells Synonyms: soluble Fas Ligand (sFasL), TNFSF6, CD95L, Apo I Ligand, APTL AA Sequence: HHHHHHHHPS PPPEKKELRK VAHLTGKSNS RSMPLEWEDT YGIVLLSGVK YKKGGLVINE TGLYFVYSKV YFRGQSCNNL PLSHKVYMRN SKYPQDLVMM EGKMMSYCTT GQMWARSSYL GAVFNLTSAD HLYVNVSELS LVNFEESQTF FGLYKL Purity: ≥ 95% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by its ability to induce cytotoxicity in Jurkat cells in the absence of any cross-linking. The ED50 for this effect is ≤ 10.0 ng/ml, corresponding to a specific activity of ≥ 1 x 105 units/mg. Calculated Molecular Weight: 17.9 kDa Accession Number: P48023 Gene ID: 356 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) crossreactivity: Human, Mouse, References PubMed SDS Búsqueda del CoA Product Line Country Of Origin: USA Not for human use. Research Interest Apoptosis Immune System TNF Superfamily product.subtitle.recentcitations Primer autor Tello-Lafoz, M Titulo Cytotoxic lymphocytes target characteristic biophysical vulnerabilities in cancer. Citar Immunity; 54(5) pg1037-1054.e7 PudMed id 33756102 Primer autor Gorbea, C Titulo A viral Sm-class RNA base-pairs with mRNAs and recruits microRNAs to inhibit apoptosis. Citar Nature; 550(7675) pg275-279 PudMed id 28976967 Primer autor Lim, M C Titulo Pathogen-induced ubiquitin-editing enzyme A20 bifunctionally shuts off NF-κB and caspase-8-dependent apoptotic cell death. Citar Cell Death and Differentiation; 24(9) pg1621-1631 PudMed id 28574503