Recombinant Human Slit2-N 0 ReviewsSubmit a Review Product Details Catalogue Number: 150-11 Description: Slit2 is a member of the Slit family that signals through the Roundabout (Robo) receptor as a repellent for axon guidance and neuronal migration, and also acts as a chemoattractant to vascular endothelial cells and a chemotaxis inhibitor for leukocytes. Slit2 is expressed primarily in the fetal lung, kidney, and adult spinal cord, and to a lesser extent in the adult adrenal gland, thyroid and trachea. Slit2 is initially synthesized as a 1499 amino acid precursor, which is subsequently cleaved into N-terminal and C-terminal fragments, designated as Slit2-N and Slit2-C respectively. The neurodevelopment-related activities, as measured by the ability to repel olfactory bulb axons and to induce branching in dorsal root ganglia axons, are contained only in the N-terminal fragment. Recombinant Human Slit2-N is a 1093 amino acid glycoprotein corresponding to the N-terminal portion of the full length Slit2 precursor, and has a calculated, theoretical molecular weight of 122.35 kDa. Due to glycosylation Slit2-N migrates at an apparent molecular weight of approximately 120.0-140.0 kDa by SDS-PAGE analysis under reducing conditions. Source: HEK293 cells Synonyms: Slit homolog 2 protein, SLIL3 AA Sequence: QACPAQCSCS GSTVDCHGLA LRSVPRNIPR NTERLDLNGN NITRITKTDF AGLRHLRVLQ LMENKISTIE RGAFQDLKEL ERLRLNRNHL QLFPELLFLG TAKLYRLDLS ENQIQAIPRK AFRGAVDIKN LQLDYNQISC IEDGAFRALR DLEVLTLNNN NITRLSVASF NHMPKLRTFR LHSNNLYCDC HLAWLSDWLR QRPRVGLYTQ CMGPSHLRGH NVAEVQKREF VCSGHQSFMA PSCSVLHCPA ACTCSNNIVD CRGKGLTEIP TNLPETITEI RLEQNTIKVI PPGAFSPYKK LRRIDLSNNQ ISELAPDAFQ GLRSLNSLVL YGNKITELPK SLFEGLFSLQ LLLLNANKIN CLRVDAFQDL HNLNLLSLYD NKLQTIAKGT FSPLRAIQTM HLAQNPFICD CHLKWLADYL HTNPIETSGA RCTSPRRLAN KRIGQIKSKK FRCSAKEQYF IPGTEDYRSK LSGDCFADLA CPEKCRCEGT TVDCSNQKLN KIPEHIPQYT AELRLNNNEF TVLEATGIFK KLPQLRKINF SNNKITDIEE GAFEGASGVN EILLTSNRLE NVQHKMFKGL ESLKTLMLRS NRITCVGNDS FIGLSSVRLL SLYDNQITTV APGAFDTLHS LSTLNLLANP FNCNCYLAWL GEWLRKKRIV TGNPRCQKPY FLKEIPIQDV AIQDFTCDDG NDDNSCSPLS RCPTECTCLD TVVRCSNKGL KVLPKGIPRD VTELYLDGNQ FTLVPKELSN YKHLTLIDLS NNRISTLSNQ SFSNMTQLLT LILSYNRLRC IPPRTFDGLK SLRLLSLHGN DISVVPEGAF NDLSALSHLA IGANPLYCDC NMQWLSDWVK SEYKEPGIAR CAGPGEMADK LLLTTPSKKF TCQGPVDVNI LAKCNPCLSN PCKNDGTCNS DPVDFYRCTC PYGFKGQDCD VPIHACISNP CKHGGTCHLK EGEEDGFWCI CADGFEGENC EVNVDDCEDN DCENNSTCVD GINNYTCLCP PEYTGELCEE KLDFCAQDLN PCQHDSKCIL TPKGFKCDCT PGYVGEHCDI DFDDCQDNKC KNGAHCTDAV NGYTCICPEG YSGLFCEFSP PMV Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by its ability to inhibit MC3T3/E1 osteoblasts cell differentiation. Calculated Molecular Weight: 122.35 kDa Accession Number: O94813 Gene ID: 9353 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) Note: 200μg is the largest vial size for this product. Larger sizes supplied as multiple 100‐200μg vials. crossreactivity: Human, Mouse References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest AIDS/HIV Angiogenesis/Cardiovascular Cancer Chemotaxis Immune System Neurobiology Wound Healing product.subtitle.recentcitations First Author McConnell, R E Title A requirement for filopodia extension toward Slit during Robo-mediated axon repulsion. Citation The Journal of Cell Biology; 213(2) pg261-74 PubMed Id 27091449 First Author Xu, F Title Whole-exome and targeted sequencing identify ROBO1 and ROBO2 mutations as progression-related drivers in myelodysplastic syndromes. Citation Nature Communications; 6 pg8806 PubMed Id 26608094 First Author Shrivastava, A Title Slit2N Inhibits Transmission of HIV-1 from Dendritic Cells to T-cells by Modulating Novel Cytoskeletal Elements. Citation Scientific Reports; 5 pg16833 PubMed Id 26582347
