Recombinant Human Sonic Hedgehog (Shh) 0 ReviewsSubmit a Review Product Details Catalogue Number: 100-45 Description: Members of the Hedgehog (Hh) family are highly conserved proteins that are widely represented throughout the animal kingdom. The three known mammalian Hh proteins, Sonic (Shh), Desert (Dhh) and Indian (Ihh), are structurally related, and share a high degree of amino acid sequence identity (e.g. Shh and Ihh are 93% identical). The biologically active form of each Hh molecule is obtained by autocatalytic cleavage of their precursor proteins, and each corresponds to approximately one half of the N-terminal portion of the precursor molecule. Although Hh proteins have unique expression patterns and distinct biological roles within their respective regions of secretion, they use the same signaling pathway and can be substituted for one another in experimental systems. Recombinant E. coli-derived Human Sonic Hedgehog is a 20.0 kDa protein consisting of 176 amino acid residues, including an N-terminal Ile-Val-Ile sequence substituted for the naturally occurring, chemically modified, Cys residue. Source: E.coli Synonyms: Hhg-1 AA Sequence: IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRALDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) cells. The expected ED50 for this effect is 0.8-1.0 μg/ml. Calculated Molecular Weight: 20 kDa Accession Number: Q15465 Gene ID: 6469 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) Note: 1mg will be provided as 2x500μg. crossreactivity: Human, Mouse References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Organoids Apoptosis Bone, Skeletal, Cartilage Immune System Neurobiology Stem Cells & Differentiation Wound Healing product.subtitle.recentcitations First Author Tang, C Title 3D Interfacial and Spatiotemporal Regulation of Human Neuroepithelial Organoids. Citation Advanced science (Weinheim, Baden-Wurttemberg, Germany); 9(22) pge2201106 PubMed Id 35667878 First Author Ngan, S Y Title Stage-Specific Generation of Human Pluripotent Stem Cell Derived Lung Models to Measure CFTR Function. Citation Current Protocols; 2(1) pge341 PubMed Id 35025140 First Author Hu, D Title Small-molecule suppression of calpastatin degradation reduces neuropathology in models of Huntington's disease Citation Nature Communications; 12(1) pg5305 PubMed Id 34489447
