Extracellular Matrix

Recombinant Human Vitronectin

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Product Details

Catalogue Number: 140-09
Description:
Recombinant Human Vitronectin

Vitronectin is a secreted glycoprotein that is synthesized in the liver. It circulates primarily in monomeric form, but can undergo conformational change to a structure that forms disulfide-linked multimers. The multimeric vitronectin can efficiently bind to, and incorporate into, the extracellular matrix. Within the matrix, vitronectin can support cell adhesion through binding to various integrins and other proteoglycans. Additionally, recombinant vitronectin can function as a chemically-defined matrix component in human embryonic stem cell renewal media. Recombinant Human Vitronectin is a 459 amino acid, single-chain, monomeric protein, which migrates at an apparent molecular weight of 75 kDa by SDS-PAGE under reducing conditions. The calculated molecular weight of Recombinant Human Vitronectin is 52.2 kDa.

Note: On December 1, 2022 this product will be discontinued. PeproTech’s Animal-Free Recombinant Human Vitronectin (Catalog# AF-140-09) can be used as a substitution for this product.

Source: HEK293 cells

Synonyms: VTN, Serum-spreading factor, V75

AA Sequence: DQESCKGRCT EGFNVDKKCQ CDELCSYYQS CCTDYTAECK PQVTRGDVFT MPEDEYTVYD DGEEKNNATV HEQVGGPSLT SDLQAQSKGN PEQTPVLKPE EEAPAPEVGA SKPEGIDSRP ETLHPGRPQP PAEEELCSGK PFDAFTDLKN GSLFAFRGQY CYELDEKAVR PGYPKLIRDV WGIEGPIDAA FTRINCQGKT YLFKGSQYWR FEDGVLDPDY PRNISDGFDG IPDNVDAALA LPAHSYSGRE RVYFFKGKQY WEYQFQHQPS QEECEGSSLS AVFEHFAMMQ RDSWEDIFEL LFWGRTSAGT RQPQFISRDW HGVPGQVDAA MAGRIYISGM APRPSLAKKQ RFRHRNRKGY RSQRGHSRGR NQNSRRPSRA TWLSLFSSEE SNLGANNYDD YRMDWLVPAT CEPIQSVFFF SGDKYYRVNL RTRRVDTVDP PYPRSIAQYW LGCPAPGHL

Purity: ≥ 95% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Recombinant Human Vitronectin promotes attachment of hESC and iPSC in serum-free, feeder conditions at 5µg/ml. See PeproTech’s hESC medium manual for further information.

Calculated Molecular Weight: 52.2 kDa

Accession Number: P04004

Gene ID: 7448

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Tan, J L
Title
Restoration of the healing microenvironment in diabetic wounds with matrix-binding IL-1 receptor antagonist
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Communications Biology; 4(1) pg422
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First Author
Briggs, D C
Title
Inter-α-inhibitor heavy chain-1 has an integrin-like 3D structure mediating immune regulatory activities and matrix stabilization during ovulation.
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The Journal of Biological Chemistry; 295(16) pg5278-5291
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First Author
Dardik, R
Title
The small tellurium-based compound SAS suppresses inflammation in human retinal pigment epithelium.
Citation
Molecular Vision; 22 pg548-62
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