Recombinant Murine HGF 0 ReviewsSubmit a Review Product Details Catalogue Number: 315-23 Description: HGF is a potent, mesenchymally-derived mitogen for mature parenchymal hepatocytes, and acts as a growth factor for a broad spectrum of tissues and cell types. HGF signals through a transmembrane tyrosine kinase receptor known as MET. Activities of HGF include the induction of cell proliferation, motility, morphogenesis, inhibition of cell growth, and enhancement of neuron survival. HGF is a crucial mitogen for liver regeneration processes, especially after partial hepatectomy and other liver injuries. Human and murine HGF are cross-reactive. Murine HGF is expressed as a linear, polypeptide-precursor glycoprotein containing 696 amino acid residues. Proteolytic processing of this precursor generates the biologically active heterodimeric form of HGF, which consists of two polypeptide chains (α-chain and β-chain) held together by a single disulfide bond resulting in formation of a biologically active heterodimer. The α-chain consists of 463 amino acid residues and four kringle domains. The β-chain consists of 233 amino acid residues. Recombinant Murine HGF is a 79.3 kDa polypeptide consisting of 696 amino acid residues. Source: (BTI-Tn-5B1-4) Hi-5 Insect cells Synonyms: Hepatocyte Growth Factor, Scatter Factor (SF), Hepatopoietin (HPTA) AA Sequence: Alpha chain: QKKRRNTLHE FKKSAKTTLT KEDPLLKIKT KKVNSADECA NRCIRNRGFT FTCKAFVFDK SRKRCYWYPF NSMSSGVKKG FGHEFDLYEN KDYIRNCIIG KGGSYKGTVS ITKSGIKCQP WNSMIPHEHS FLPSSYRGKD LQENYCRNPR GEEGGPWCFT SNPEVRYEVC DIPQCSEVEC MTCNGESYRG PMDHTESGKT CQRWDQQTPH RHKFLPERYP DKGFDDNYCR NPDGKPRPWC YTLDPDTPWE YCAIKTCAHS AVNETDVPME TTECIQGQGE GYRGTSNTIW NGIPCQRWDS QYPHKHDITP ENFKCKDLRE NYCRNPDGAE SPWCFTTDPN IRVGYCSQIP KCDVSSGQDC YRGNGKNYMG NLSKTRSGLT CSMWDKNMED LHRHIFWEPD ASKLNKNYCR NPDDDAHGPW CYTGNPLIPW DYCPISRCEG DTTPTIVNLD HPVISCAKTK QLRBeta chain: VVNGIPTQTT VGWMVSLKYR NKHICGGSLI KESWVLTARQ CFPARNKDLK DYEAWLGIHD VHERGEEKRK QILNISQLVY GPEGSDLVLL KLARPAILDN FVSTIDLPSY GCTIPEKTTC SIYGWGYTGL INADGLLRVA HLYIMGNEKC SQHHQGKVTL NESELCAGAE KIGSGPCEGD YGGPLICEQH KMRMVLGVIV PGRGCAIPNR PGIFVRVAYY AKWIHKVILT YKL Purity: ≥ 95% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by the dose-dependent stimulation of the proliferation of mouse IMCD3 cells using a concentration range of 10-20 ng/ml. Calculated Molecular Weight: 79.3 kDa Accession Number: Q08048 Gene ID: 15234 Note: 250μg is the largest vial size for this product. Larger sizes supplied as multiple 250μg vials. crossreactivity: Human, Mouse, Rat, Salamander References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Organoids Angiogenesis/Cardiovascular Cancer Immune System Neurobiology Stem Cells & Differentiation Wound Healing product.subtitle.recentcitations First Author Zhou, Y Title Genetically engineered distal airway stem cell transplantation protects mice from pulmonary infection. Citation EMBO Molecular Medicine; 12(1) pge10233 PubMed Id 31782624 First Author Mukai, H Title PKN3 is the major regulator of angiogenesis and tumor metastasis in mice. Citation Scientific Reports; 6 pg18979 PubMed Id 26742562 First Author Li, B Title Adult Mouse Liver Contains Two Distinct Populations of Cholangiocytes. Citation Stem Cell Reports; 9(2) pg478-489 PubMed Id 28689996
