Growth Factors & Cytokines

Recombinant Murine IL-3

Product Details

Catalogue Number: 213-13
Description:

IL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages.  Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoiesis, phagocytosis, and antibody-mediated cellular cytotoxicity.  Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles.  Many of the IL-3 activities depend upon co-stimulation with other cytokines.  IL-3 is a species-specific, variably glycosylated cytokine.  Recombinant Murine IL-3 is a 15.1 kDa globular protein containing 135 amino acid residues.

Source: E.coli

Synonyms: MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor

AA Sequence: MDTHRLTRTL NCSSIVKEII GKLPEPELKT DDEGPSLRNK SFRRVNLSKF VESQGEVDPE DRYVIKSNLQ KLNCCLPTSA NDSALPGVFI RDLDDFRKKL RFYMVHLNDL ETVLTSRPPQ PASGSVSPNR GTVEC

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of murine NFS-60 cells is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.

This product has been tested against the WHO standard. International Unit information can be found here.

Calculated Molecular Weight: 15.1 kDa

Accession Number: P01586

Gene ID: 16187

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Kauts, M
Title
Rapid Mast Cell Generation from Gata2 Reporter Pluripotent Stem Cells.
Citation
Stem Cell Reports; 11(4) pg1009-1020
PubMed Id
First Author
Guo, S
Title
Complex oncogene dependence in microRNA-125a-induced myeloproliferative neoplasms.
Citation
Proceedings of the National Academy of Sciences of the United States of America; 109(41) pg16636-41
PubMed Id
First Author
Bettini, M L
Title
MAP kinase phosphatase activity sets the threshold for thymocyte positive selection.
Citation
Proceedings of the National Academy of Sciences of the United States of America; 104(41) pg16257-62
PubMed Id

product.subtitle.productreviews

Existing reviews
Better than any other brand
Used to treat cells but used different company products and they weren't got delivered it as fast as this one
From:  Cansu | Date:  28/07/2020
Recombinant Murine IL-3
We use this product to make stock dilutions, and store at -20. until we need to prep more murine media. They had always worked well post thaw
From:  Nichole | Date:  26/08/2020