Recombinant Murine sCD40 Ligand 0 ReviewsSubmit a Review Product Details Catalogue Number: 315-15 Description: CD40, a member of the TNF receptor family, is a cell surface protein expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells. Signaling though CD40 plays an important role in the proliferation and differentiation of B cells, and is critical for immunoglobulin (Ig) class switching. The membrane-anchored CD40 Ligand is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40L leads to "immunodeficiency with hyper-IgM", a disease characterized by failure to produce IgG, IgA and IgE. The soluble form of CD40L is an 18kDa protein comprising the entire TNF homologous region of CD40L and is generated in vivo by an intracellular proteolytic processing of the full length CD40L. Recombinant Murine CD40L is a soluble 16.4 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CD40L. Source: E.coli Synonyms: soluble CD40 Ligand, TNFSF5, TRAP, CD154, Gp39, T-BAM AA Sequence: MQRGDEDPQI AAHVVSEANS NAASVLQWAK KGYYTMKSNL VMLENGKQLT VKREGLYYVY TQVTFCSNRE PSSQRPFIVG LWLKPSSGSE RILLKAANTH SSSQLCEQQS VHLGGVFELQ AGASVFVNVT EASQVIHRVG FSSFGLLKL Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by its ability to induce TNF-α and MIP-1α production by murine splenocytes. The expected ED50 for this effect is < 0.1 µg/ml. Calculated Molecular Weight: 16.4 kDa Accession Number: P27548 Gene ID: 21947 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) crossreactivity: Human, Mouse, Mouse + Virus, Rat References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Organoids Angiogenesis/Cardiovascular Apoptosis Immune System Inflammation TNF Superfamily Allergy Transplantation product.subtitle.recentcitations First Author Chen, M Title TRIM14 Promotes Noncanonical NF-κB Activation by Modulating p100/p52 Stability via Selective Autophagy. Citation Advanced science (Weinheim, Baden-Wurttemberg, Germany); 7(1) pg1901261 PubMed Id 31921549 First Author Andreani, V Title Cochaperone Mzb1 is a key effector of Blimp1 in plasma cell differentiation and β1-integrin function. Citation Proceedings of the National Academy of Sciences of the United States of America; 115(41) pgE9630-E9639 PubMed Id 30257949 First Author Chen, J Title The RNA-binding protein ROD1/PTBP3 cotranscriptionally defines AID-loading sites to mediate antibody class switch in mammalian genomes. Citation Cell Research; 28(10) pg981-995 PubMed Id 30143796
