Recombinant Rat SCF 0 ReviewsSubmit a Review Product Details Catalogue Number: 400-22 Description: SCF is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor. SCF and c-Kit are essential for the survival, proliferation and differentiation of hematopoietic cells committed to the melanocyte and germ cell lineages. Human SCF manifests low activity on murine cells, while murine and rat SCF are fully active on human cells. The human SCF gene encodes for a 273 amino acid transmembrane protein, which contains a 25 amino acid N-terminal signal sequence, a 189 amino acid extracellular domain, a 23 amino acid transmembrane domain, and a 36 amino acid cytoplasmic domain. The secreted soluble form of SCF is generated by proteolytic processing of the membrane anchored precursor. Recombinant Rat SCF is an 18.4 kDa polypeptide containing 165 amino acid residues, which corresponds to the sequence of the secreted soluble form of SCF. Source: E.coli Synonyms: Stem Cell Factor, c-Kit Ligand, Mast Cell Growth Factor (MGF), Steel Factor AA Sequence: MQEICRNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV THLSVSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVAC MEENAPKNVK ESLKKPETRN FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by the dose-dependent stimulation of the proliferation of the human TF-1 cells. The expected ED50 for this effect is 20 - 40 ng/ml. Calculated Molecular Weight: 18.4 kDa Accession Number: P21581 Gene ID: 60427 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) crossreactivity: Human, Mouse, Rat References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Cell Culture Immune System Stem Cells & Differentiation product.subtitle.recentcitations First Author Barbier, V Title Endothelial E-selectin inhibition improves acute myeloid leukaemia therapy by disrupting vascular niche-mediated chemoresistance. Citation Nature Communications; 11(1) pg2042 PubMed Id 32341362 First Author Rangel, E B Title Kidney-derived c-kit Citation Scientific Reports; 8(1) pg14723 PubMed Id 30283057 First Author Khanna-Gupta, A Title Up-regulation of translation eukaryotic initiation factor 4E in nucleophosmin 1 haploinsufficient cells results in changes in CCAAT enhancer-binding protein α activity: implications in myelodysplastic syndrome and acute myeloid leukemia. Citation The Journal of Biological Chemistry; 287(39) pg32728-37 PubMed Id 22851180
