Growth Factors & Cytokines

Recombinant Rat SCF

0 ReviewsSubmit a Review

Product Details

Catalogue Number: 400-22
Description:
Recombinant Rat SCF

SCF is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor. SCF and c-Kit are essential for the survival, proliferation and differentiation of hematopoietic cells committed to the melanocyte and germ cell lineages. Human SCF manifests low activity on murine cells, while murine and rat SCF are fully active on human cells. The human SCF gene encodes for a 273 amino acid transmembrane protein, which contains a 25 amino acid N-terminal signal sequence, a 189 amino acid extracellular domain, a 23 amino acid transmembrane domain, and a 36 amino acid cytoplasmic domain. The secreted soluble form of SCF is generated by proteolytic processing of the membrane anchored precursor. Recombinant Rat SCF is an 18.4 kDa polypeptide containing 165 amino acid residues, which corresponds to the sequence of the secreted soluble form of SCF.

Source: E.coli

Synonyms: Stem Cell Factor, c-Kit Ligand, Mast Cell Growth Factor (MGF), Steel Factor

AA Sequence: MQEICRNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV THLSVSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVAC MEENAPKNVK ESLKKPETRN FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by the dose-dependent stimulation of the proliferation of the human TF-1 cells. The expected ED50 for this effect is 20 - 40 ng/ml.

Calculated Molecular Weight: 18.4 kDa

Accession Number: P21581

Gene ID: 60427

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Barbier, V
Title
Endothelial E-selectin inhibition improves acute myeloid leukaemia therapy by disrupting vascular niche-mediated chemoresistance.
Citation
Nature Communications; 11(1) pg2042
PubMed Id
First Author
Rangel, E B
Title
Kidney-derived c-kit
Citation
Scientific Reports; 8(1) pg14723
PubMed Id
First Author
Khanna-Gupta, A
Title
Up-regulation of translation eukaryotic initiation factor 4E in nucleophosmin 1 haploinsufficient cells results in changes in CCAAT enhancer-binding protein α activity: implications in myelodysplastic syndrome and acute myeloid leukemia.
Citation
The Journal of Biological Chemistry; 287(39) pg32728-37
PubMed Id