Anticorps Polyclonaux Purifiés par Affinité à l’Antigène

Anti-Human BD-3

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Product Details

Catalogue Number: 500-P241

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBD-3. Anti-Human BD-3 specific antibody was purified by affinity chromatography employing immobilized hBD-3 matrix.

Immunogen: E.coli derived Recombinant Human BD-3 (PeproTech catalog# 300-52)

Sandwich ELISA: To detect hBD-3 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human BD-3 (500-P241Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBD-3.

Anti-Human BD-3 Sandwich ELISA

Western Blot: To detect hBD-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBD-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human BD-3 Western Blot Reduced Anti-Human BD-3 Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Jin, G
Title
An antimicrobial peptide regulates tumor-associated macrophage trafficking via the chemokine receptor CCR2, a model for tumorigenesis.
Citation
PLoS ONE; 5(6) pge10993
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First Author
Kalus, A A
Title
Association of a genetic polymorphism (-44 C/G SNP) in the human DEFB1 gene with expression and inducibility of multiple beta-defensins in gingival keratinocytes.
Citation
BMC Oral Health; 9 pg21
PubMed Id
First Author
Johnston, A
Title
IL-1F5, -F6, -F8, and -F9: a novel IL-1 family signaling system that is active in psoriasis and promotes keratinocyte antimicrobial peptide expression.
Citation
Journal of immunology (Baltimore, Md. : 1950); 186(4) pg2613-22
PubMed Id