Anticorps Polyclonaux Purifiés par Affinité à l’Antigène

Anti-Human IGF-I

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Product Details

Catalogue Number: 500-P11

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IGF-I. Anti-Human IGF-I-specific antibody was purified by affinity chromatography employing immobilized Human IGF-I matrix.

Immunogen: E.coli derived Recombinant Human IGF-I (PeproTech catalog# 100-11)

Sandwich ELISA: To detect hIGF-I by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human IGF-I (500-P11Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-I.

Anti-Human IGF-I Sandwich ELISA

Western Blot: To detect hIGF-I by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-I is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human IGF-I Western Blot Reduced Anti-Human IGF-I Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Annibalini, G
Title
The intrinsically disordered E-domains regulate the IGF-1 prohormones stability, subcellular localisation and secretion.
Citation
Scientific Reports; 8(1) pg8919
PubMed Id
First Author
Vanderkerken, K
Title
Insulin-like growth factor-1 acts as a chemoattractant factor for 5T2 multiple myeloma cells.
Citation
Blood; 93(1) pg235-41
PubMed Id
First Author
Jeschke, M G
Title
Exogenous liposomal IGF-I cDNA gene transfer leads to endogenous cellular and physiological responses in an acute wound.
Citation
American journal of physiology. Regulatory, integrative and comparative physiology; 286(5) pgR958-66
PubMed Id