Anticorps Polyclonaux Purifiés par Affinité à l’Antigène

Anti-Human IL-12

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Product Details

Catalogue Number: 500-P154G

Source: Polyclonal Goat

Preparation: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hIL12. Anti-Human IL-12 specific antibody was purified by affinity chromatography employing immobilized hIL12 matrix.

Immunogen: CHO cells derived Recombinant Human IL-12 p70 (PeproTech catalog# 200-12)

Sandwich ELISA: To detect hIL-12 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human IL-12 (500-P154GBt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-12.

Anti-Human IL-12 Sandwich ELISA

Western Blot: To detect hIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-12 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human IL-12 Western Blot Reduced Anti-Human IL-12 Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Bouhaddou, M
Title
The Global Phosphorylation Landscape of SARS-CoV-2 Infection.
Citation
Cell; 182(3) pg685-712.e19
PubMed Id
First Author
Fong, L E
Title
Systems analysis of latent HIV reversal reveals altered stress kinase signaling and increased cell death in infected T cells.
Citation
Scientific Reports; 7(1) pg16179
PubMed Id
First Author
Woo, J S
Title
CRACR2A-Mediated TCR Signaling Promotes Local Effector Th1 and Th17 Responses.
Citation
Journal of immunology (Baltimore, Md. : 1950); 201(4) pg1174-1185
PubMed Id