Anticorps Polyclonaux Purifiés par Affinité à l’Antigène

Anti-Human IL-17A (Polyclonal Rabbit)

0 ReviewsSubmit a Review

Product Details

Catalogue Number: 500-P07

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-17A. Anti-Human IL-17A specific antibody was purified by affinity chromatography employing immobilized hIL-17 matrix.

Immunogen: E.coli derived Recombinant Human IL-17A (PeproTech catalog# 200-17)

Sandwich ELISA: To detect hIL-17A by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human IL-17A (500-P07Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-17A.

Anti-Human IL-17A Sandwich ELISA

Western Blot: To detect hIL-17A by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-17A is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human IL-17A Western Blot Reduced Anti-Human IL-17A Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Lu, S
Title
IL-17A, but not IL-17F, is indispensable for airway vascular remodeling induced by exaggerated Th17 cell responses in prolonged ovalbumin-challenged mice.
Citation
Journal of immunology (Baltimore, Md. : 1950); 194(8) pg3557-66
PubMed Id
First Author
Brucklacher-Waldert, V
Title
Phenotypical characterization of human Th17 cells unambiguously identified by surface IL-17A expression.
Citation
Journal of immunology (Baltimore, Md. : 1950); 183(9) pg5494-501
PubMed Id
First Author
Peters, T L
Title
Neither IL-17A mRNA nor IL-17A protein are detectable in Langerhans cell histiocytosis lesions.
Citation
Molecular therapy : the journal of the American Society of Gene Therapy; 19(8) pg1433-9
PubMed Id