Anticorps Polyclonaux Purifiés par Affinité à l’Antigène

Anti-Human IL-6 (Polyclonal Rabbit)

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Product Details

Catalogue Number: 500-P26
Description:
Anti-Human IL-6 (Polyclonal Rabbit)
 

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure recombinant Human IL-6. Anti-Human IL-6 specific antibody was purified by affinity chromatography employing immobilized Human IL-6 matrix.

Immunogen: E.coli derived Recombinant Human IL-6 (PeproTech catalog# 200-06)

Sandwich ELISA: To detect Human IL-6 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human IL-6 (500-P26Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-6.

Anti-Human IL-6 Sandwich ELISA

Western Blot: To detect Human IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-6 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human IL-6 Western Blot Reduced Anti-Human IL-6 Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Blanco-Melo, D
Title
Imbalanced Host Response to SARS-CoV-2 Drives Development of COVID-19.
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Cell; 181(5) pg1036-1045.e9
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First Author
Zhang, R
Title
Cancer-associated fibroblasts enhance tumor-associated macrophages enrichment and suppress NK cells function in colorectal cancer.
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Cell Death & Disease; 10(4) pg273
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Chong, H C
Title
Regulation of epithelial-mesenchymal IL-1 signaling by PPARbeta/delta is essential for skin homeostasis and wound healing.
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The Journal of Cell Biology; 184(6) pg817-31
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