Anticorps Polyclonaux Purifiés par Affinité à l’Antigène

Anti-Human MCP-1 (CCL2)

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Product Details

Catalogue Number: 500-P34

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMCP-1(MCAF). Anti-Human MCP-1(MCAF) specific antibody was purified by affinity chromatography employing immobilized hMCP-1(MCAF) matrix.

Immunogen: E.coli derived Recombinant Human MCP-1 (CCL2) (PeproTech catalog# 300-04)

Sandwich ELISA: To detect hMCP-1(MCAF) by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human MCP-1(MCAF) (500-P34Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMCP-1(MCAF).

Anti-Human MCP-1 (CCL2) Sandwich ELISA

Western Blot: To detect hMCP-1(MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-1(MCAF) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human MCP-1 (CCL2) Western Blot Reduced Anti-Human MCP-1 (CCL2) Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Pontejo, S M
Title
Two glycosaminoglycan-binding domains of the mouse cytomegalovirus-encoded chemokine MCK-2 are critical for oligomerization of the full-length protein.
Citation
The Journal of Biological Chemistry; 292(23) pg9613-9626
PubMed Id
First Author
Fatrai, S
Title
KRAS(G12V) enhances proliferation and initiates myelomonocytic differentiation in human stem/progenitor cells via intrinsic and extrinsic pathways.
Citation
The Journal of Biological Chemistry; 286(8) pg6061-70
PubMed Id
First Author
Kim, M S
Title
MCP-1 is induced by receptor activator of nuclear factor-{kappa}B ligand, promotes human osteoclast fusion, and rescues granulocyte macrophage colony-stimulating factor suppression of osteoclast formation.
Citation
The Journal of Biological Chemistry; 280(16) pg16163-9
PubMed Id