Anti-Human Midkine 0 ReviewsSubmit a Review Product Details Catalogue Number: 500-P171 Source: Polyclonal Rabbit Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMidkine. Anti-Human Midkine specific antibody was purified by affinity chromatography employing immobilized hMidkine matrix. Immunogen: E.coli derived Recombinant Human Midkine (PeproTech catalog# 450-16) Sandwich ELISA: To detect hMidkine by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human Midkine (500-P171Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMidkine. Western Blot: To detect hMidkine by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMidkine is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Note: Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information. crossreactivity: Human, Mouse References SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Angiogenesis/Cardiovascular Neurobiology Stem Cells & Differentiation product.subtitle.recentcitations First Author Kuo, A H Title Recruitment of insulin receptor substrate-1 and activation of NF-kappaB essential for midkine growth signaling through anaplastic lymphoma kinase. Citation Oncogene; 26(6) pg859-69 PubMed Id 16878150 First Author Zhou, Z Title NGPF2 triggers synaptic scaling up through ALK-LIMK-cofilin-mediated mechanisms. Citation Cell Reports; 36(7) pg109515 PubMed Id 34407403 First Author You, Z Title Midkine is a NF-kappaB-inducible gene that supports prostate cancer cell survival. Citation BMC Medical Genomics; 1 pg6 PubMed Id 18275606
