Anticorps Polyclonaux Purifiés par Affinité à l’Antigène

Anti-Human Nanog

Product Details

Catalogue Number: 500-P236

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hNanog. Anti-Human Nanog specific antibody was purified by affinity chromatography employing immobilized hNanog matrix.

Immunogen: E.coli derived Recombinant Human Nanog (PeproTech catalog# 120-21)

Sandwich ELISA: To detect hNanog by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human Nanog (500-P236Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNanog.

Anti-Human Nanog Sandwich ELISA

Western Blot: To detect hNanog by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNanog is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human Nanog Western Blot Reduced Anti-Human Nanog Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Takemitsu, H
Title
Comparison of bone marrow and adipose tissue-derived canine mesenchymal stem cells.
Citation
BMC Veterinary Research; 8 pg150
PubMed Id
First Author
Ramos-Ibeas, P
Title
Pluripotency and X chromosome dynamics revealed in pig pre-gastrulating embryos by single cell analysis.
Citation
Nature Communications; 10(1) pg500
PubMed Id
First Author
Kong, Q
Title
RE1-silencing Transcription Factor (REST) Is Required for Nuclear Reprogramming by Inhibiting Transforming Growth Factor β Signaling Pathway.
Citation
The Journal of Biological Chemistry; 291(53) pg27334-27342
PubMed Id

product.subtitle.productreviews

Existing reviews
bovine embryos and derived embryonic stem cells
This product works really good in in vitro bovine embryos and embryonic stem cells derived on MEFs. Perfect to validate pluripotency
From:  Nicolás | Date:  09/07/2020