Anticorps Polyclonaux Purifiés par Affinité à l’Antigène

Anti-Murine IL-10

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Product Details

Catalogue Number: 500-P60

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-10. Anti-Murine IL-10 specific antibody was purified by affinity chromatography employing immobilized mIL-10 matrix.

Immunogen: E.coli derived Recombinant Murine IL-10 (PeproTech catalog# 210-10)

Sandwich ELISA: To detect mIL-10 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Murine IL-10 (500-P60Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-10.

Anti-Murine IL-10 Sandwich ELISA

Western Blot: To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Murine IL-10 Western Blot Reduced Anti-Murine IL-10 Western Blot Unreduced


Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Country Of Origin: USA

Not for human use.

Research Interest


First Author
García-Prieto, E
Resistance to bleomycin-induced lung fibrosis in MMP-8 deficient mice is mediated by interleukin-10.
PLoS ONE; 5(10) pge13242
PubMed Id
First Author
Hu, L
Depletion of ID3 enhances mesenchymal stem cells therapy by targeting BMP4 in Sjögren's syndrome.
Cell death & disease; 11(3) pg172
PubMed Id
First Author
Maldonado Galdeano, C
Impact of a probiotic fermented milk in the gut ecosystem and in the systemic immunity using a non-severe protein-energy-malnutrition model in mice.
BMC Gastroenterology; 11 pg64
PubMed Id