Anticorps Polyclonaux Purifiés par Affinité à l’Antigène

Anti-Rat GRO/KC (CXCL1)

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Product Details

Catalogue Number: 500-P74

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure recombinant Rat GRO/KC. Anti-Rat GRO/KC specific antibody was purified by affinity chromatography employing immobilized Rat GRO/KC matrix.

Immunogen: E.coli derived Recombinant Rat GRO/KC (CXCL1) (PeproTech catalog# 400-10)

Sandwich ELISA: To detect Rat GRO/KC by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Rat GRO/KC (500-P74Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat GRO/KC.

Anti-Rat GRO/KC (CXCL1) Sandwich ELISA

Western Blot: To detect Rat GRO/KC by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat GRO/KC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Rat GRO/KC (CXCL1) Western Blot Reduced Anti-Rat GRO/KC (CXCL1) Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Rat
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Chandrasekar, B
Title
Ischemia-reperfusion of rat myocardium activates nuclear factor-KappaB and induces neutrophil infiltration via lipopolysaccharide-induced CXC chemokine.
Citation
Circulation; 103(18) pg2296-302
PubMed Id
First Author
Nabah, Y N
Title
Angiotensin II induces neutrophil accumulation in vivo through generation and release of CXC chemokines.
Citation
Circulation; 110(23) pg3581-6
PubMed Id
First Author
Mateo, T
Title
A critical role for TNFalpha in the selective attachment of mononuclear leukocytes to angiotensin-II-stimulated arterioles.
Citation
Blood; 110(6) pg1895-902
PubMed Id