Anticorps Polyclonaux Purifiés par Affinité à l’Antigène

Anti-Rat IL-4

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Product Details

Catalogue Number: 500-P94

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure recombinant Rat IL-4. Anti-Rat IL-4 specific antibody was purified by affinity chromatography employing immobilized Rat IL-4 matrix.

Immunogen: E.coli derived Recombinant Rat IL-4 (PeproTech catalog# 400-04)

Sandwich ELISA: To detect Rat IL-4 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Rat IL-4 (500-P94Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-4.

Anti-Rat IL-4 Sandwich ELISA

Western Blot: To detect Rat IL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat IL-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Rat IL-4 Western Blot Reduced Anti-Rat IL-4 Western Blot Unreduced


Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Country Of Origin: USA

Not for human use.

Research Interest


First Author
Mateo, T
A critical role for TNFalpha in the selective attachment of mononuclear leukocytes to angiotensin-II-stimulated arterioles.
Blood; 110(6) pg1895-902
PubMed Id
First Author
Schif-Zuck, S
Coadministration of plasmid DNA constructs encoding an encephalitogenic determinant and IL-10 elicits regulatory T cell-mediated protective immunity in the central nervous system.
Journal of immunology (Baltimore, Md. : 1950); 177(11) pg8241-7
PubMed Id
First Author
Wildbaum, G
Tr1 cell-dependent active tolerance blunts the pathogenic effects of determinant spreading.
The Journal of Clinical Investigation; 110(5) pg701-10
PubMed Id