Anticorps Polyclonaux Purifiés par Affinité à l’Antigène

Anti-Rat TNF-α

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Product Details

Catalogue Number: 500-P72

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure recombinant Rat TNF-α. Anti-Rat TNF-α specific antibody was purified by affinity chromatography employing immobilized Rat TNF-α matrix.

Immunogen: E.coli derived Recombinant Rat TNF-α (PeproTech catalog# 400-14)

Sandwich ELISA: To detect Rat TNF-α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Rat TNF-α (500-P72Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat TNF-α.

Anti-Rat TNF-alpha Sandwich ELISA

Western Blot: To detect Rat TNF-α by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat TNF-α is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Rat TNF-alpha Western Blot Reduced Anti-Rat TNF-alpha Western Blot Unreduced


Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Country Of Origin: USA

Not for human use.

Research Interest


First Author
Mao, L
Epothilone B impairs functional recovery after spinal cord injury by increasing secretion of macrophage colony-stimulating factor.
Cell death & disease; 8(11) pge3162
PubMed Id
First Author
Foright, R M
Compensatory eating behaviors in male and female rats in response to exercise training.
American journal of physiology. Regulatory, integrative and comparative physiology; 319(2) pgR171-R183
PubMed Id
First Author
Aires, I D
Blockade of microglial adenosine A
Glia; 67(5) pg896-914
PubMed Id