Biotinylated Anti-Human BAFF (Polyclonal Goat) 0 ReviewsSubmit a Review Product Details Catalogue Number: 500-P163GBT Description: Source: Polyclonal Goat Preparation: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hBAFF. Anti-Human BAFF specific antibody was purified by affinity chromatography and then biotinylated. Immunogen: E.coli derived Recombinant Human BAFF (PeproTech catalog# 310-13) Sandwich ELISA: To detect hBAFF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human BAFF (500-P163G) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hBAFF. Western Blot: To detect hBAFF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBAFF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Note: 1mg will be provided as 2x500μg. crossreactivity: Human References SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Organoids Apoptosis Immune System Stem Cells & Differentiation TNF Superfamily product.subtitle.recentcitations First Author Sanders, J F Title Plasma levels of soluble interleukin 2 receptor, soluble CD30, interleukin 10 and B cell activator of the tumour necrosis factor family during follow-up in vasculitis associated with proteinase 3-antineutrophil cytoplasmic antibodies: associations with disease activity and relapse. Citation Annals of the Rheumatic Diseases; 65(11) pg1484-9 PubMed Id 16504995
