Anticorps Polyclonaux Biotinylés

Biotinylated Anti-Human IL-4

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Product Details

Catalogue Number: 500-P24BT

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-4. Anti-Human IL-4 specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: E.coli derived Recombinant Human IL-4 (PeproTech catalog# 200-04)

Sandwich ELISA: To detect hIL-4 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human IL-4 (500-P24) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hIL-4.

Western Blot: To detect hIL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Biotinylated Anti-Human IL-4 Western Blot Reduced Biotinylated Anti-Human IL-4 Western Blot Unreduced

Note:

1mg will be provided as 2x500μg.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Blanco-Melo, D
Title
Imbalanced Host Response to SARS-CoV-2 Drives Development of COVID-19.
Citation
Cell; 181(5) pg1036-1045.e9
PubMed Id