Anticorps Polyclonaux Biotinylés

Biotinylated Anti-Human IL-8 (CXCL8)

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Product Details

Catalogue Number: 500-P28BT

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure recombinant Human IL-8. Anti-Human IL-8 specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: E.coli derived Recombinant Human IL-8 (CXCL8) (72 a.a.) (PeproTech catalog# 200-08M)

Sandwich ELISA: To detect Human IL-8 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human IL-8 (500-P28) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human IL-8.

Biotinylated Anti-Human IL-8 (CXCL8) Sandwich ELISA

Western Blot: To detect Human IL-8 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-8 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Biotinylated Anti-Human IL-8 (CXCL8) Western Blot Reduced Biotinylated Anti-Human IL-8 (CXCL8) Western Blot Unreduced

Note:

1mg will be provided as 2x500μg.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Vanheule, V
Title
The Positively Charged COOH-terminal Glycosaminoglycan-binding CXCL9(74-103) Peptide Inhibits CXCL8-induced Neutrophil Extravasation and Monosodium Urate Crystal-induced Gout in Mice.
Citation
The Journal of Biological Chemistry; 290(35) pg21292-304
PubMed Id
First Author
Rius, C
Title
Trans- but not cis-resveratrol impairs angiotensin-II-mediated vascular inflammation through inhibition of NF-κB activation and peroxisome proliferator-activated receptor-gamma upregulation.
Citation
Journal of immunology (Baltimore, Md. : 1950); 185(6) pg3718-27
PubMed Id
First Author
Øynebråten, I
Title
Characterization of a novel chemokine-containing storage granule in endothelial cells: evidence for preferential exocytosis mediated by protein kinase A and diacylglycerol.
Citation
Journal of immunology (Baltimore, Md. : 1950); 175(8) pg5358-69
PubMed Id