Biotinylated Anti-Human sRANK Ligand (Polyclonal Rabbit) 0 AvisSubmit a Review Détails du Produit Catalog Number: 500-P133BT Description: Source: Polyclonal Rabbit Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hsRANKL. Anti-Human sRANKL specific antibody was purified by affinity chromatography and then biotinylated. Immunogen: E.coli-derived, 20.0 kDa Recombinant Human sRANK Ligand (PeproTech catalog# 310-01) Sandwich ELISA: To detect hsRANKL by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human sRANKL (500-P133) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hsRANKL. Western Blot: To detect hsRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANKL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Note: 1mg will be provided as 2x500μg. crossreactivity: Human References SDS Recherche de CoA Product Line Country Of Origin: USA Not for human use. Research Interest Apoptosis Bone, Skeletal, Cartilage Immune System TNF Superfamily product.subtitle.recentcitations Premier Auteur Tso, G H Titre Phagocytosis of apoptotic cells modulates mesenchymal stem cells osteogenic differentiation to enhance IL-17 and RANKL expression on CD4+ T cells. Citation Stem cells (Dayton, Ohio); 28(5) pg939-54 Id PubMed 20222014 Premier Auteur Grimaud, E Titre Receptor activator of nuclear factor kappaB ligand (RANKL)/osteoprotegerin (OPG) ratio is increased in severe osteolysis. Citation The American Journal of Pathology; 163(5) pg2021-31 Id PubMed 14578201