Cytokines RUO

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mJE(MCP-1). Anti-Murine JE(MCP-1) specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: E.coli derived Recombinant Murine JE/MCP-1 (CCL2) (PeproTech catalog# 250-10)

Sandwich ELISA: To detect mJE(MCP-1) by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Murine JE(MCP-1) (500-P113) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mJE(MCP-1).

Western Blot: To detect mJE(MCP-1) by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mJE(MCP-1) is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Note:

1mg will be provided as 2x500μg.