Biotinylated Anti-Rat EGF 0 AvisSubmit a Review Détails du Produit Catalog Number: 500-P277BT Description: Source: Polyclonal Rabbit Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat EGF (Rat Epidermal Growth Factor). Anti-Rat EGF specific antibody was purified by affinity chromatography and then biotinylated. Immunogen: E.coli derived Recombinant Rat EGF (PeproTech catalog# 400-25) Sandwich ELISA: To detect Rat EGF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Rat EGF (500-P277) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat EGF. Western Blot: To detect Rat EGF by Western Blot analysis this antibody can be used at a concentration of 0.5 - 1.0 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat EGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Note: 1mg will be provided as 2x500μg. SDS Recherche de CoA Product Line Country Of Origin: USA Not for human use. Research Interest Organoids Angiogenesis/Cardiovascular Cancer Cell Culture Inflammation Neurobiology Proliferation Stem Cells & Differentiation Wound Healing