Anticorps Polyclonaux Biotinylés

Biotinylated Anti-Rat RANTES (CCL5)

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Product Details

Catalogue Number: 500-P78BT

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat RANTES. Anti-Rat RANTES specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: E.coli-derived, 7.9 kDa Recombinant Rat RANTES (CCL5) (PeproTech catalog# 400-13)

Sandwich ELISA: To detect Rat RANTES by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Rat RANTES (500-P78) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat RANTES.

Biotinylated Anti-Rat RANTES (CCL5) Sandwich ELISA

Western Blot: To detect Rat RANTES by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg /ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat RANTES is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Biotinylated Anti-Rat RANTES (CCL5) Western Blot Reduced Biotinylated Anti-Rat RANTES (CCL5) Western Blot Unreduced

Note:

1mg will be provided as 2x500μg.

crossreactivity:
Rat
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Abu-Taha, M
Title
Menopause and ovariectomy cause a low grade of systemic inflammation that may be prevented by chronic treatment with low doses of estrogen or losartan.
Citation
Journal of immunology (Baltimore, Md. : 1950); 183(2) pg1393-402
PubMed Id