Produced from sera of rabbits pre-immunized with highly pure recombinant Rat TNF-α. Anti-Rat TNF-α specific antibody was purified by affinity chromatography and then biotinylated.
E.coli-derived, 17.3 kDa Recombinant Rat TNF-α (PeproTech catalog# 400-14)
To detect Rat TNF-α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Rat TNF-α (500-P72) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat TNF-α.
To detect Rat TNF-α by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat TNF-α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
1mg will be provided as 2x500μg.
Not for human use.