Produced from sera of rabbits immunized with highly pure Recombinant Human HB-EGF. Anti-Human HB-EGF-specific antibody was purified by affinity chromatography and then biotinylated.
E.coli-derived, 9.7 kDa Recombinant Human HB-EGF (PeproTech Catalog# 100-47)
To detect Human HB-EGF by sandwich ELISA (using 100μl/well), a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human HB-EGF (500-P329) as a capture antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human HB-EGF.
To detect Human HB-EGF by Western Blot analysis, this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human HB-EGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.