Pre-Coated ELISA Kits

IFN-gamma is an immunoregulatory cytokine produced primarily by activated Th2 cells, and also by mast cells and NK cells. Targeted deletion of IFN-γ in mice resulted in impaired Th2 cell development and indicated an important role for IFN-gamma in the expulsion of gastrointestinal parasites. IFN-γ exerts anti-inflammatory effects on monocytes and macrophages and it inhibits the expression of inflammatory cytokines such as IL-1β, TNF-α, IL-6 and IL-8. IFN-γ has also been shown to enhance B cell proliferation and to induce isotype switching, resulting in increased production of IgE. Blocking of IFN-γ activity inhibits the pathophysiology of asthma. Human and murine IFN-γ are cross-species reactive. The BioGems Human IFN-γ Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human IFN-γ with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for IFN-γ. It contains E.coli-expressed recombinant Human IFN-γ. The detection antibody is a biotinylated goat polyclonal antibody specific for IFN-γ. The kit is analytically validated with ready to use reagents.