Pre-Coated ELISA Kits

Macrophage migration inhibitory factor (MIF) is a small secreted protein that can act as a pleiotropic pro-inflammatory cytokine, as well as an enzyme. MIF pro-inflammatory activity can be initiated by signaling through CD74 and CD44, resulting in the secretion of TNF-a, IL-1, IL-6, IL-8, and various MMPs. The enzymatic activity of MIF is characterized by its ability to act as a tautomerase, capable of catalyzing the keto-to-enol isomerization of keto-phenylpyruvate and L-dopachrome. It appears as though MIF catalytic activity is dependent upon a trimeric configuration and a free N-terminal proline residue. The BioGems Human MIF Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure MIF with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for MIF. It contains E.coli-expressed recombinant MIF. The detection antibody is a biotinylated goat polyclonal antibody specific for MIF. The kit is analytically validated with ready to use reagents.