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Pre-Coated ELISA Kits

Biogems

Human TNF-α Pre-Coated ELISA Kit

Détails du Produit

Catalog Number: BGK01375
Description:

TNF-α is a pleiotropic pro-inflammatory cytokine secreted by various cells, including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands, and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells, and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, autoimmune diseases, rheumatoid arthritis, inflammation, and diabetes. Human and murine TNF-α demonstrate significant cross-species reactivity. TNF-α exists in two forms; a type II transmembrane protein, and a mature soluble protein. The TNF-α transmembrane protein is proteolitically cleaved to yield a soluble, biologically active, 17 kDa TNF-α, which forms a non-covalently linked homotrimer in solution. The BioGems Human TNF-α Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human TNF-α with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for TNF-α. It contains E.coli-expressed recombinant Human TNF-α. The detection antibody is a biotinylated goat polyclonal antibody specific for TNF-alpha. The kit is analytically validated with ready to use reagents.

Sandwich ELISA:

Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<1 pg/ml 
Range:
 15.6 pg/mL- 1,000 pg/mL
Specificity:
Natural and recombinant Human TNF-a
Uniprot:
 P01375
Interference:
No detectable cross-reactivity with other relevant proteins

Note:

Product supplied by Biogems

Research Interest

Country Of Origin: USA

Not for human use.