PeproTech’s PeproGrow™ hESC Media products are comprised of complete, chemically-defined formulations designed for the feeder-free expansion and maintenance of both human embryonic stem cells (hESCs) and human induced pluripotent stem cells (iPSCs). Intended for the culturing of hESCs and iPSCs in the undifferentiated, pluripotent state (Nanog+/Lin28+/Tra-1-60+/SSEA1-/SSEA4+/Oct4+), these formulations demonstrate less than 15% spontaneous differentiation as indicated by immunofluorescent staining and flow cytometry. These proprietary formulations were designed and developed by PeproTech in collaboration with the Stem Cell Training Course at Rutgers University. Each PeproGrow™ hESC Medium Kit includes a bottle of basal medium and a separate, lyophilized component of PeproTech’s recombinant growth factors. Additional companion products are available separately and include the Animal-Free Human Vitronectin Matrix and Buffer Kit (catalog number AF-VMB-220), and the Cell Passaging/Non-Enzymatic Detachment Buffer (catalog number CPD-125). PeproTech’s Animal-Free Human Vitronectin Matrix and Buffer Kit can be used following either a surface-coating or premix method.
PeproGrow™ hESC Plus Medium Kit is an insulin-inclusive formulation ideal for use when switching from other insulin-inclusive media products.